Dual Regulation of the lin-14 Target mRNA by the lin-4 miRNA
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چکیده
منابع مشابه
Dual Regulation of the lin-14 Target mRNA by the lin-4 miRNA
microRNAs (miRNAs) are ∼22 nt regulatory RNAs that in animals typically bind with partial complementarity to sequences in the 3' untranslated (UTR) regions of target mRNAs, to induce a decrease in the production of the encoded protein. The relative contributions of translational inhibition of intact mRNAs and degradation of mRNAs caused by binding of the miRNA vary; for many genetically validat...
متن کاملPosttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans.
During C. elegans development, the temporal pattern of many cell lineages is specified by graded activity of the heterochronic gene Lin-14. Here we demonstrate that a temporal gradient in Lin-14 protein is generated posttranscriptionally by multiple elements in the lin-14 3'UTR that are regulated by the heterochronic gene Lin-4. The lin-14 3'UTR is both necessary and sufficient to confer lin-4-...
متن کاملRegulation by let-7 and lin-4 miRNAs Results in Target mRNA Degradation
MicroRNAs (miRNAs) are approximately 22 nucleotide RNAs that negatively regulate the expression of protein-coding genes. In a present model of miRNA function in animals, miRNAs that form imperfect duplexes with their targets inhibit protein expression without affecting mRNA levels. Here, we report that in C. elegans, regulation by the let-7 miRNA results in degradation of its lin-41 target mRNA...
متن کاملThe C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.
lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1). We have cloned the C. elegans lin-4 locus by chromosomal walking and transformation rescue. We used the C. elegans clone to isolate ...
متن کاملThe lin-4 microRNA
MicroRNAs (miRNAs) are full of surprises. The more we study these ~22-nucleotide non-coding RNAs, the more we realize how little is understood about this mode of gene regulation. 1 Case in point: lin-4, the founding member of the miRNA superfamily discovered over 20 years ago. Thousands of miRNAs have since been discovered in plant, animal, and viral genomes. They direct important cellular proc...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0075475